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| Registros recuperados: 11 | |
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| Álvarez,Elba; Iglesia,Aleika. |
| Information about the characterization and diagnostic of wild fire was compiled. This bacterial disease, exotic for Cuba, is caused by Pseudomonas syringae pv. tabaci. The species Pseudomonas syringae is traditionally divided into pathovars depending on the host range and symptoms. For its study, different phenotypic and genotypic methods are used. Such methods allow the traditional taxonomic confirmation of this pathovar within the species. Serological and molecular techniques such as: ELISA tests, immunofluorescence, polymerase chain reaction (PCR) are also used for the characterization and detection of the bacterium. Such techniques are very useful and sensible for the diagnostic. |
| Tipo: Journal article |
Palavras-chave: Pseudomonas syringae pv. tabaci; Rep-PCR; Pathogenicity; Serology. |
| Ano: 2007 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522007000300001 |
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| Naranjo Feliciano,Eber; Yglesia Lozano,Aleika; García,Armando; Martínez Zubiaur,Yamila. |
| Se estableció la diversidad genética de 30 aislados cubanos de Ralstonia. solanacearum, obtenidos de papa y tomate y pertenecientes a los biovares 1 y 2, mediante la amplificación de las secuencias consenso repetitivas intergénicas de enterobacterias (rep-PCR) con los cebadores ERIC. El método permitió distinguir 11 genotipos entre los aislados cubanos. Las agrupaciones primarias alcanzadas, mediante el análisis de conglomerados, se correspondieron con el biovar de los aislamientos, mientras que las subdivisiones encontradas dentro de cada grupo, tuvieron relación con el hospedero del cual fueron obtenidos. Los aislados de papa mostraron un coeficiente de similitud del 37,2% y la presencia de variantes específicas para cada región geográfica dentro de cada... |
| Tipo: Journal article |
Palavras-chave: Ralstonia solanacearum; Diversidad genética; Rep-PCR; Papa; Tomate. |
| Ano: 2015 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1010-27522015000100009 |
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| Pinheiro,Cassia Renata; Amorim,Julie Anne Espíndola; Diniz,Leandro Eugenio Cardamone; Silva,Adriano Márcio Freire da; Talamini,Viviane; Souza Júnior,Manoel Teixeira. |
| O objetivo deste trabalho foi identificar isolados brasileiros de Ralstonia solanacearum quanto a filotipos e sequevares, determinar sua diversidade genética, realizar a associação da estrutura genética do patógeno com sua classificação e origem geográfica e identificar um marcador molecular para a diagnose do moko-da-bananeira. Um grupo de 33 isolados de R. solanacearum, da coleção da Embrapa Tabuleiros Costeiros, coletado de diversos hospedeiros, foi caracterizado por meio de PCR em sequência palindrômica extragênica repetitiva (rep-PCR) e RAPD. Deste grupo, 19 perteciam à raça 2 do patógeno e 14 à raça 1, e 15 isolados eram associados à cultura da bananeira. Os filotipos e sequevares foram caracterizados e determinados por PCR Multiplex. Verificou-se... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Marcador molecular; Moko-da-bananeira; Murcha bacteriana; Rep-PCR; Variabilidade genética. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2011000600004 |
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| Costa,Samara B.; Ferreira,Marisa A.S.V.; Lopes,Carlos A.. |
| Foi avaliada a diversidade de isolados de Ralstonia solanacearum obtidos de tomateiro e de outras hospedeiras com sintomas de murcha bacteriana na região amazônica. Os isolados foram identificados quanto à biovar e separados em graus de virulência em plantas de tomate, pimentão e chicória da Amazônia (Eryngium foetidum). Dos 70 isolados, 53 pertenciam à biovar 1, quatro à biovar N2 e 13 à biovar 3, confirmando a predominância da biovar 1 em tomateiro no Estado do Amazonas. O agrupamento dos isolados mostrou três classes distintas de virulência em tomate, sendo 44,3% dos isolados altamente virulentos, 37,1% medianamente virulentos e 18,6% fracamente virulentos. O agrupamento em pimentão classificou 20% de isolados como altamente virulentos, 27,1% como... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Murcha bacteriana; Lycopersicon esculentum; Seqüências repetitivas; Rep-PCR. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000400002 |
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| Tejada,TS; Silva,CSJ; Lopes,NA; Silva,DT; Agostinetto,A; Silva,EF; Menezes,DB; Timm,CD. |
| ABSTRACT Salmonella species have been isolated from various kinds of food and are accountable for outbreaks of foodborne diseases in humans. This study aimed at identifying the similarities between the DNA profiles of Salmonella isolated from chicken feces, chicken products, and human feces in southern Brazil. Six hundred samples were collected (200 from chicken products, 200 from broiler chicken feces, and 200 from human feces) and tested for the presence of Salmonella. Isolates proven to be Salmonella compatible by biochemical and serological tests were tested by the Polymerase Chain Reaction. Their DNA profiles were then analyzed by PFGE and rep-PCR. Salmonella was isolated from 16 out of 600 analyzed samples, with Schwarzengrund serotype presenting the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mbandaka; Schwarzengrund; Panama; PFGE; Rep-PCR. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400693 |
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| Oliveira,Sílvia Dias de; Bessa,Marjo Cadó; Santos,Luciana Ruschel dos; Cardoso,Marisa Ribeiro de Itapema; Brandelli,Adriano; Canal,Cláudio Wageck. |
| In order to study the epidemiology of Salmonella Enteritidis outbreaks and determine the source of contamination so that a recurrence can be avoided, detailed characterization is necessary. Thus, the purpose of this study was to verify whether rep-PCR was able to discriminate among Salmonella Enteritidis isolates. Phage typing, detection of virulence genes and antimicrobial resistance testing were also associated to rep-PCR results. One hundred and two S. Enteritidis isolates from broiler carcasses, food, human, pigs, poultry-related samples, and nine isolates from other countries were genotypically typed by REP-PCR, ERIC-PCR and BOX-PCR, collectively called rep-PCR. Phage typing, detection of virulence genes and antimicrobial resistance testing were also... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella Enteritidis; Rep-PCR; Phage typing; Antimicrobial resistance; Virulence genes. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400025 |
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| Borges,Wardsson Lustrino; Prin,Yves; Ducousso,Marc; Le Roux,Christine; Faria,Sergio Miana de. |
| Abstract Little is known regarding how the increased diversity of nitrogen-fixing bacteria contributes to the productivity and diversity of plants in complex communities. However, some authors have shown that the presence of a diverse group of nodulating bacteria is required for different plant species to coexist. A better understanding of the plant symbiotic organism diversity role in natural ecosystems can be extremely useful to define recovery strategies of environments that were degraded by human activities. This study used ARDRA, BOX-PCR fingerprinting and sequencing of the 16S rDNA gene to assess the diversity of root nodule nitrogen-fixing bacteria in former bauxite mining areas that were replanted in 1981, 1985, 1993, 1998, 2004 and 2006 and in a... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhizobia; Rep-PCR; Cluster analysis; Nodules; Mining. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000200314 |
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| Koc,Melih; Cokmus,Cumhur; Cihan,Arzu Coleri. |
| Abstract Thermophilic 32 isolates and 20 reference bacilli were subjected to Rep-PCR and ITS-PCR fingerprinting for determination of their genotypic diversity, before screening lipase activities. By these methods, all the isolates and references could easily be differentiated up to subspecies level from each other. In screening assay, 11 isolates and 7 references were found to be lipase producing. Their extracellular lipase activities were measured quantitatively by incubating in both tributyrin and olive oil broths at 60 °C and pH 7.0. During the 24, 48 and 72-h period of incubation, the changes in the lipase activities, culture absorbance, wet weight of biomass and pH were all measured. The activity was determined by using pNPB in 50 mM phosphate buffer... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Endospore-forming bacilli; Screening; Thermostable lipase; ITS-PCR; Rep-PCR. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401065 |
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| Registros recuperados: 11 | |
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